I am working in SPR based biosensor. When polymer (polyethylene Glycol) mixed with cells, How the interaction among them? Does the polimer can only bind with living cells? (The cells that have been mixed, contains live and dead cells).
Dear Mr. Devi,
In a biosensor, the bioreceptor is designed to interact with the specific analyte of interest to produce an effect measurable by the transducer. High selectivity for the analyte among a matrix of other chemical or biological components is a key requirement of the bioreceptor. While the type of biomolecule used can vary widely, biosensors can be classified according to common types bioreceptor interactions involving: anitbody/antigen, enzymes/ligands, nucleic acids/DNA, cellular structures/cells, or biomimetic materials
It is important to emphasis that bioreceptor and analyte (in your case cell) can interact spesifically and efficiently.
So, you need to make sure that Polyethylene Glycol (PEG) is really work with your analyte (cell).
In case, to determine is Biosensor can detect the living cell, is depend on your cell sample.
It is better to know the mechanism of interaction between sample and bioreceptor.
In same case, living cell keep in their condition to ensure the interactian still working.
By the way, I attach a Journal, hopefully it can help.
http://link.springer.com/article/10.1007/s11356-012-1329-7
Cheers,
Indra
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