How stable is DNA in strong alkaline conditions?

06 March 2019 0 8K Report

How stable is DNA in strong alkaline conditions such as 1 M KOH at 37 C? If it is not stable, it is because of loss of the bases or cleavage of the backbone? Anyone has done detailed experiments on this? Thanks.

Badges
Science topic

Similar topics
Social Science
Media
Journalism

More Qing Dai's questions See All

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How to enrich pig excreta for increasing nutrient quality organically ?

Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.

09 August 2024 5,604 2 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 266 3 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,217 4 View

Leaf area of tomato ?

Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of a tomato? Can you explain with an example and what is the substitution of Ln and ln?

06 August 2024 2,508 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,786 4 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,804 3 View

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,018 3 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,724 2 View

What are the limitations and challenges of using machine learning for predicting concrete compressive strength in practical applications?

Machine learning (ML) has shown great potential in predicting the compressive strength of concrete, an important property for structural engineering. However, its practical application comes with...

03 August 2024 2,545 2 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,628 1 View

What should I keep in mind when designing a gene construct when trying to co-transform to increase protein expression?

I am having trouble expressing a protein and have heard that co-transforming it can improve stability and increase expression, so I am looking to try this experiment. I only need the kinase domain...

08 July 2024 2,935 5 View

How can I collect Zirfon membrane for alkaline water electrolysis?

I am doing research in the United Sates America. So, I will pay in US dollar. Could you please tell me how can i get Zirfon membrane? Thank you.

24 June 2024 7,667 0 View

How to choose the time and acid-base environment for the Clayson Schmidt reaction?

I want to replicate the third step, but there seems to be no reaction in an alkaline environment. However, sodium hydroxide is used in the literature. Should I switch to an acidic environment

05 June 2024 9,358 1 View

How could I analyze the results of caspase-glo 3/7 assay?

I have done caspase-glo 3/7 assay which measures caspase activity after cleavage and generation of luminescent signal produced by luciferase. However,after I got the results, I am not sure how to...

02 June 2024 7,833 2 View

Are mulching & ZT treatments useful in agricultural soils particularly alkaline calcareous nature & low in OM & RT minimizing loss of OM?

Are mulching and zero tillage treatments useful in agricultural soils particularly alkaline calcareous nature and low in organic matter and reducing tillage minimizing the loss of organic matter?

02 June 2024 1,409 4 View

Can I purify mononucleosome from T-cell using a kit followed by SEC superdex 200 10/300 column chromatography for CryoEM ?

I am planning to purify the mononucleosome for cryoEM study. I have seen, people assemble the nucleosome from recombinantly expressed histones and then providing DNA sequence to it. Isn't this...

19 May 2024 7,169 1 View

What tool can predict E. coli protease cleavage sites?

I am observing cleavage in my construct expressed in E. coli and attempting to identify the responsible proteases. Mass spectrometry analysis has revealed the sizes of the fragments, enabling...

12 May 2024 6,540 2 View

What failure mechanisms lead to the observation of cleavage and flat facets in fractographs of tensile samples?

05 May 2024 6,530 5 View

Between Ni foam and Glassy carbon electrode, which current collector better to study the materials for OER and HER ?

Between Ni foam and Glassy carbon electrode, which current collector is better for studying the nanomaterials/ nanocomposites for OER and HER in the alkaline medium?

31 March 2024 8,170 1 View