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Does anyone know the exact BS concentration of the Zymo DNA methylation gold kit? or is there anyway to measure the BS concentration by titration? Thanks.
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It is known that DNase I can make random single-stranded cuts in the DNA. NEB's fragments used a second, single-strand-specific enzyme that recognizes nicked sites then cleaves the second strand....
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How stable is DNA in strong alkaline conditions such as 1 M KOH at 37 C? If it is not stable, it is because of loss of the bases or cleavage of the backbone? Anyone has done detailed experiments...
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ADP sodium salt is commercially available and I followed the literature to use Dowex-50-W ion exchange column to convert the sodium from to H+ form and neutralize it by adding Bu4NOH to pH 6.2...
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How to purify ATP in a large quantity, like 1 gram? Is it possible to run a silica gel column instead of a ion-exchange column? Thanks.
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