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Questions related from Qing Dai
Hi, we are looking for commercial DNA polymerase that can be used for amplify the post-BS treatment DNA for long template. Although several commercial DNA polymerase can be used to amplify post-BS...
21 November 2024 2,152 0 View
Does anyone know the exact BS concentration of the Zymo DNA methylation gold kit? or is there anyway to measure the BS concentration by titration? Thanks.
06 May 2024 8,929 1 View
It is known that DNase I can make random single-stranded cuts in the DNA. NEB's fragments used a second, single-strand-specific enzyme that recognizes nicked sites then cleaves the second strand....
07 April 2023 3,986 2 View
How stable is DNA in strong alkaline conditions such as 1 M KOH at 37 C? If it is not stable, it is because of loss of the bases or cleavage of the backbone? Anyone has done detailed experiments...
06 March 2019 8,101 0 View
ADP sodium salt is commercially available and I followed the literature to use Dowex-50-W ion exchange column to convert the sodium from to H+ form and neutralize it by adding Bu4NOH to pH 6.2...
25 January 2018 7,266 0 View
How to purify ATP in a large quantity, like 1 gram? Is it possible to run a silica gel column instead of a ion-exchange column? Thanks.
10 November 2017 6,063 5 View
Hi there, We have been trying to use the EM-seq from NEB to construct libraries. When using lambda DNA without 5mC as a model DNA, we found the conversion rate is usually less than 99%. The...
01 January 1970 4,397 0 View
