The peptide TFLLR is a Par-1 receptor agonist. It is used for activating astrocytes in the CNS.
I would like to know how specific it is.
Hi, me and my team mates are currently planning to pursue the topic of Activated Carbon from Coconut as a Water Filter. And we would like to ask for your suggestions and knowledge on what specific...
22 February 2021 6,569 3 View
21 February 2021 7,561 4 View
I would like to ask what other properties of water (pH and such) shall be analyzed and evaluated to test the effectiveness of activated carbon from filtering the contaminants present in the water?
20 February 2021 9,422 8 View
Hi! Me and my team mates are currently new in doing a research study. We do not have any previous experiences in research. And we just wanted to know what type of experts (the field they in) can...
19 February 2021 9,596 4 View
Hello, I've build a Conv1D model to classify items into 6 categories (from 0 to 5). (simplified example) I use therefore a CrossEntropy loss function that penalizes the errors with high...
19 January 2021 3,199 1 View
Hello, would you please share the codes for Base Isolation of structures using Double Concave Friction Pendulum Bearings? I am doing a related research but as I am just a beginner in MATLAB, I...
18 January 2021 6,683 1 View
07 January 2021 6,360 2 View
The SCI questionnaire for measuring domains of self-care appears in several places on the internet and scantly in the literature. There are no published validation studies or...
02 December 2020 6,848 1 View
I recently just found out about this statistical software and am interested to learn more about how this can be applied in marine fish ecology. Any leads on relevant literature would be most helpful.
15 November 2020 6,960 2 View
Hello, For one of my projects I would like to test a Live RNA imaging using molecular beacons such as in this paper : Molecular Beacon-based Detection and Isolation of Working-ty... As we...
28 October 2020 9,457 2 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?
28 February 2021 1,254 3 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
23 February 2021 4,014 3 View
I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...
22 February 2021 10,099 5 View
I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...
21 February 2021 4,537 3 View
This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...
21 February 2021 564 3 View
I was differentiate hESC to astrocyte. In the second week of differentiation, I stained my cell with s100beta and GFAP. I expected that the staining site of GFAP would come from the cytoplasm,...
15 February 2021 8,982 1 View
Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...
11 February 2021 562 6 View
"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...
11 February 2021 6,628 9 View