Greetings all,
I'm interested in studying the zeta size of a couple of nanoparticles solutions I have tested on simple acute toxicity assays. Those NPs were suspended in three or four different organisms media, with some salts diluted in them. The Zetasizer used was the model "Nano ZS" (from Malvern Panalytical) and the software was "Zetasizer Software".
I have analyzed different batches concerning different concentrations utilized of each tested suspension. The problem I'm having while analyzing the data is that I lost the saved analysis files from the department PC that was coupled to the device. I was left with the "raw" data (values) for each reading done by the device concerning each batch I analyzed. I wanted to present the results like the ones in Fig.1 but I guess that that is automatically done through the software, right? Can I establish a range of sizes and do it manually? How should I treat the data?
If something's not clear, wrong, or there is some info. missing to judge the problem, please don't refrain to ask. Thanks in advance for your time and attention.
Best regards,
Pedro Nunes
Is it possible to import these data as a Table format?
Sorting these data in different columns would allow you to work with some analysis software program like Origin.
Pedro Nunes
I'm not sure what you mean by raw data - these are the photons being counted by the APD and then being stored in a correlator whose first output is the correlogram. What you appear to show are results with no idea as to how they were obtained. The most robust outputs from DLS (ISO 22412 & ASTM E2490) are the z-average and the polydispersity index (PI). What do you mean by 'average diameter'? Volume? Intensity? What does the size of the 'corresponding medium' mean?
There are the ways to refine the data and process it using the ascii files.
I see no ‘raw data’ that can be reprocessed. Just a table of numbers... Correct me if I am wrong...
Is there a .dts or .zet file available?
Hello Pedro Nunes
the raw data should have been stored on the computer, by default in the folder My Documents - Malvern Instruments - Zetasizer - Measurement Data
and the file should have the extension " .dts " . Even if the software was uninstalled, the data files should still be there on that computer. You can copy the data, and download and install the software again (free) and then open the data file.
https://www.materials-talks.com/blog/2014/08/19/faq-how-can-i-submit-a-data-file-to-the-help-desk/
A list of the downloadable software versions for the Zetasizer Nano is at https://www.materials-talks.com/blog/2015/03/09/how-to-get-the-latest-software-for-the-zetasizer-nano/
I have a small doubt. In DLS, I am always getting multiple random peaks (Intensity vs size data) for water or buffer even if I am passing it through 0.45 um filter. Then, based on what ground should I neglect this artifacts.
Swagatam Barman
You are measuring noise. DLS does not work like (for example) laser diffraction or UV-VIS. You do not take or subtract a ‘background’. Your results of filtered DI water are meaningless. Understand the basics of the measurement...
Further you should post a separate question on RG to avoid confusing this thread.
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