Haley Luu

4 Questions 1 Answers 0 Followers

Questions related from Haley Luu

How should I treat A549 cells with Pseudomonas LPS?

I am trying to treat A549 cells with Pseudomonas LPS to stimulate antimicrobial peptide expression. How should I go about this? I am not sure where to start or what to do.

06 July 2022 2,342 1 View

How do you design a probe for smFISH?

I am planning on performing smFISH of changed antimicrobial peptide genes on lung or cultured cells to visualize where the lung peptides are expressed. I am trying to figure out how to design a...

07 February 2022 8,899 0 View

How do you use a loading control in a western blot?

I am trying to run a western blot on A549 cells with beta-actin as the loading control and alpha-SMA as the protein of interest. However, both beta-actin and alpha-SMA have similar kDas. How would...

05 December 2021 7,557 3 View

How should I make a protein lysate from cultured A549 cells?

I am trying to prepare my cells in order to perform a Western Blot on them. I was wondering if anyone has had any experience with creating protein lysates for these cells.

07 August 2021 1,224 2 View