some paper reported taking just after mixing some reported after drying
Just take simple absorbance of your nanoparticles solution synthesized from plant extract by UV Visible spectrophotometer. It will be better if you take absorbance of extract too in dilute solution
dear humayun
you can deposit the nanoparticles on any substrate such as glass and you can take absorbance under Drs (diffused reflectance spectroscopy).
you can take simple absorbance of metal nanoparticles solution synthesized from plant extract by UV Visible spectrophotometer.
For UV VIS spectrsocopy, essentially it doesn't matter as long as you use the appropriate reference for subtraction (e.g. a blank glass slide is the reference if you deposit them on a glass slide, so that you get the absorption spectrum of the matter you are interested in). E.g. in your case the plant extract could be used as a reference (if using cuvettes to hold a solution).
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