Hello, everyone,

  I am trying to do single cell transcriptome with plant ovlue tissues. To isolate the specific cell, I hvae to embed the samples in parafin and use laser microdissection to get single cell samples. But I am confused about the RNA lysis buffer type, which can release the RNA from the cut “dead cell” but will mot affect the reverse transcription effiency. For mamal cells, there are only plasmembrane in the cell outlayer. So Triton-X 100 is always uesd to broke cell and release RNA. While plant cell has cell wall, which is hard to be digested. So how should I chose the buffer that will not affect the later reverse transcription step? 

Besides, the sample is embeded in parrafin and it will be cut in to 10um thin sections, which will be thinner than the cell diameter.So will it be easier to use simple buffer like Triton-X 100 to release the RNA from the cell? I am really confused about this problem and hope someone can give me some proffesional suggestions.

If you have some information about RNA kit for single cell and it has no colum purification step, please let me know. Thank you!!