ethical dilemmas surrounding the use of CRISPR-Cas9
how to fix this error, from phq_readin : error # 1 DFPT with the Blochl correction is not implemented I am attaching my ph.in file.
23 July 2024 4,649 2 View
Kindly confirm how to distinguish glia cells and neurons using cresyl violet staining. Also, using cresyl violet nissl staining how to identify the apoptotic neurons ? Does this stain stains...
18 July 2024 2,420 0 View
Kindly confirm can we do MTT assay for quantification of apoptosis. Is MTT Assay a valid assay to quantify apoptotic cells ? What about LDH assay ?..how is it better for apoptosis quantification...
08 July 2024 5,482 2 View
We want to purchase few units
26 June 2024 3,068 0 View
I needed help to get the published final version of a research article titled: "Alterations in placental long chain polyunsaturated fatty acid metabolism in human intrauterine growth...
11 June 2024 3,120 2 View
I am trying to measure telomere length in human DNA samples through qPCR. In the protocol, they have not included extension step. Can anybody explain how a qPCR can work without an extension step?...
31 May 2024 514 2 View
Im looking for the Cloud Technology research guide in mangalore. Can anyone recommend some names on which we discuss ?
28 May 2024 2,865 1 View
Quality Infrastructure ( QI ) has been established across the globe to create a mechanism for independent third party assessment of products, services and processes. It plays a pivotal role at the...
16 May 2024 4,703 10 View
Satellite images are available for various bandwidths. How can we reap benefits of AI to classify and recognise the status of water bodies? How can we predict the availability of quality water for...
16 May 2024 3,523 2 View
There are numerous cooking oils are available in international markets for mass consumption at an affordable prices. Due to health benefits, can we make available the Olive Oil at reasonable...
08 May 2024 4,526 3 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...
22 July 2024 7,073 2 View
I'm working on a project that involves analyzing a new dataset, and I'm at the stage of selecting the most appropriate machine learning algorithm. The dataset consists of both numerical and...
22 July 2024 6,097 7 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
I have attempted various methods, including homozygous gene disruption and CRISPR-Cas9, but have not achieved the desired results. It appears that the gene of interest is situated in a...
15 July 2024 3,977 1 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hi, I'm a graduate student, college of nursing, chungnam national university in Daejeon. The topic of my thesis is "Quality Assessment of Tools for Evaluating Acute Postoperative Pain." I have...
10 July 2024 3,048 1 View
I am looking for a CRISPR protocol for application in leishmania strains
08 July 2024 9,732 1 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
