How remove serum media by using phosphate buffer saline any one please explain the whole process?

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Adam B Shapiro

The growth medium is removed from the flask with a pipet. The saline is added to the flask, swirled around, then removed. If it is important to remove every last bit of serum, repeat the saline wash. Now the washed cells are ready for whatever comes next in your procedure.

Bhaskara Rao Simma

Thank you Mr. Adam B Shapiro for your valuable information....