How to clone pcDNA vector in SH-SY5Y cell line?

Dear all, How I can clone two genes in pcDNA vector, simultaneously (one of them is target gene with 500 bp length and one of them is GFP). Also, how we can transfect the vector into SH-SY5Y cell...

12 November 2020 6,122 3 View

I am looking for a text book on quantum theory which is suitable for mathematicians. Does anyone have a suggestion (instead of B C Hall's one)?

I mean a book which is written in math texts style. I saw Hall's book but I am looking for a book whuch has more details.

30 October 2020 1,636 6 View

How to simulate MSE walls for dynamic FE analyses?

Hi colleagues, Because of the complexity of modelling geogrid layers and their interfaces which can cause convergence problem in FE analyses, I have seen in practice they model the whole MSE wall...

14 August 2020 4,649 3 View

How I can see contact stress in my analysis result in abaqus ?

I want to report contact stress in my result, is that cpress (contact pressure) ? what is the different between contact stress and contact pressure? !

27 July 2020 3,767 3 View

Is it possible to simulate tip-sample interaction during Scanning Probe Microscopy in Abaqus? and if not which software do you recommend?

I want to simulate this interaction in Abaqus but I heard it may be possible only in Comsol, considering the samples are measured in Nano scale, thanks

01 April 2020 8,313 3 View

Does industrial scale Hydrometallurgical purification of Silicon exist?

There are several papers describing different hydro-metallurgical purification routes of Metallurgical Si to Solar Si through alloying, doping, nano sized grinding,... and finally leaching in...

28 March 2020 2,201 3 View

Why TiO2 powder earnd from sulfate process turns yellow after anealing?

Tio2 powder is earned after sulfate process by solving illmanite in the sulfuric acid and a purity of up 95% of TiO2 is gained . why the color of Tio2 turns yellow after 2h annealing in 900 oC...

07 February 2020 5,769 3 View

How to fine grind material in a narrow band particle size distribution?

Does anybody have ideas to mill Silica powder to micron size particles to a narrow particle size distribution (PSD)? For example grinding with planetary ball mill makes a wide PSD which is not...

19 December 2019 5,431 2 View

Hi all, could you please recommend a good review paper on the scheduling of renewable energy resources (RESs) in microgrids?

I would be very thankful if anyone could recommend some good review papers about the scheduling or economic dispatch of RESs in the microgrids. The assessment of the greenhouse gasses in the...

05 December 2019 6,335 3 View

Diffused dye front on SDS-PAGE, any suggestions on what the problem might be?

I ran these 15% gels for 90 minutes under 200 volts ( gel ran too slow), ladder looked very condense on the top and the dye front was somehow diffused at the bottom of the gel. I am sure about the...

16 April 2019 6,092 5 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

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01 March 2021 3,905 5 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View