What is the problem with my siRNA transfection?

I tried a pool of 3 siRNA transfection in Hela cells using RNAiMAX but all of of them failed. 100nM conc siRNA and opti-mem were used at 40~50% cell confluency and then 6 hour later, added DMEM...

02 September 2020 9,096 4 View

Why is methylene blue commonly used to measure an active surface area of nanoparticles?

Among the dyes, why is methylene blue commonly used to measure an active surface area of nanoparticles(especially graphene derivatives)? I try to find the reason, but I cannot find it. Could you...

11 March 2020 2,963 4 View

What is the meaning of this peak in small angle scattering 1D pattern?

In small angle neutron scattering SAS (actually small angle neutron scattering, SANS), I obtained a 1D pattern showing a peak which is located over 1 inverse angstrom. The actual peak position is...

23 December 2019 2,523 2 View

How can I increase the yield of this compound?

Hi guys, I want to synthesize the compound in the below picture. (Ref from Chem. Commun., 2017,53, 3189-3192 ) Its synthetic procedure seems gabriel synthesis using...

06 March 2018 6,385 3 View

Why does DMSO show quintet in 1H NMR?

Hi guys, I learned that dmso peak in 1H NMR caused by proton exchange. So the peak at 2.5 ppm indicates dmso-d5 not d6 Also the dmso which have more exchanged proton like d4 or others can be shown...

04 March 2018 8,655 3 View

Why brine(saturated NaCl solution) is better than water in extratction?

Hi, I've learned extraction using brine. The reason that I learned was increasing polarity and removing water in organic phase. Then, how this can happen and why brine is better than pure...

10 February 2018 5,516 3 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

What are your alternative data collection methods during the pandemic? What are the pros and cons?

Dear colleagues We are exploring possible data collection methods for environment and behavior research in the context of architectural environments. Have you conducted any Post Occupancy...

16 February 2021 2,575 4 View

Why do we need to neutralize the charge of the system by adding ions before we proceed to energy minimization step in GROMACS ?

I am learning MD simulations using GROMACS and while I was doing a protein in water MD simulation a doubt raised. I was following the steps mentioned on the tutorials page and it said The system...

15 February 2021 5,514 4 View

Any way to separate out fluorescent channels from a composite image?

Hi there, I've been using the EVOS Auto II microscope for some time now and usually I will save the transmitted light/bright field image and the images from fluorescent channels as separate...

15 February 2021 546 3 View