How one could extract 100% (or near to 100%) DNA and RNA from a known bacterial biomass and quantify?

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ICRP annual dose limit for occupational worker averaged over a period of 5 years is 20mSv. Is it according to the ALARA principle?

Working in an FDG production lab for around 20 years. Does the annual ICRP dose limit of 20mSv/Year avegraged over 5 years (and in a single year upto 50mSv) is following the ALARA principle. Does...

07 December 2020 404 5 View

Vaccine for Coronavirus?

I thought the last couple of days and think that if we know the source of coronavirus then it will help in one or another way. Suppose / for example, if the virus came from snake or mouse then...

11 February 2020 4,893 17 View

Is there any type citation of Ormosia fordiana Oliv.?

I am interested to know if there is any type citation of this name in Fl. Cambodge, Laos & Vietnam in vol. 23 (1987). If cited, then how it is cited?

11 January 2020 460 1 View

I am trying to do retroviral transduction using platE cells but its not giving good efficiency.Can somebody suggest ways to increase efficiency ?

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08 December 2019 3,649 3 View

Can anyone share the mathematical equations to compute the values of following parameters of a PMSG for a given value of system voltage (eg.V=120)?

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24 November 2019 8,269 1 View

Is it possible that the peak current of an electrochemical process increases at pH 5, decreases at pH 6 and increases at pH7 further?

In one of our electrochemical experiments, we observed that the peak current suddenly decreased at pH 6 and then again increased at pH 7. Does this relates to the stability of the complex at pH 5...

21 November 2019 7,548 2 View

How can I ask for using less RAM in High-Performance Computing Ssytem?

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17 November 2019 2,757 3 View

What is sampling error ?

By using Yamane's formula the required sample size was 250; now can I select 300 samples for performing a logistic regression ?

19 September 2019 6,202 8 View

How to resolve issues of Atmospheric Correction of Sentinel 2 data (acquired in 2015) using SNAP 6.0 (sen2cor plugin)?

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24 June 2018 9,620 17 View

Have you seen the attached publication?

On UGC approved list of journals

25 March 2018 2,308 4 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to quantitative analize the data of TNBS assay for gelatin?

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How to simultaneously perform multiple biochemical assays?

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01 March 2021 6,999 3 View

How to accurately quantify chitin?

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01 March 2021 1,843 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

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01 March 2021 8,169 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

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28 February 2021 4,949 3 View

RIA. How to measure?

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Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

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27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Isam Eldin Hussein Elgailani

Please look at the following below links which may help you in your analysis:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597165/pdf/bioengineering-03-00007.pdf http://www.readcube.com/articles/10.3389/fmicb.2015.00476

Thanks

Subir Nandy

Thank you, Sir (Isam Eldin Hussein Elgailani). But I'm not sure if the method could extract 100% DNA and RNA. I will try and see.