Could the presence of these abs produce a severly reduced levels of ferritin in serum and within cells?
We would like to collaborate with a specialist in the field of medicinal chemistry for a copyediting of a written article including synthesis, biological evaluation and in silico studies. The work...
09 January 2024 658 3 View
I'm trying to obtain xylose crystals from the aqueous phase of an acid hydrolysis-derived process. After evaporating the water, we performed an ethanol extraction in cold. It was supposed to yield...
18 May 2023 6,282 2 View
for example i have written ten books but they counted only four or i have directed ten thesis but they computed only one...
26 March 2023 7,528 2 View
If there is a racemic mixture, should I carry DFT calculations of both enantiomers separately? And if there is a significant difference between the theoretical results, can I hypothesize which...
29 December 2022 3,907 3 View
I am performing a meta-analysis of risk factors odds ratios including only adjusted OR. Some papers report only p-values of both univariate and multivariate analyses, and sufficient data to...
11 March 2022 5,973 1 View
I am developing a paper about flow of gases through restriction orifice plates and I have found in Google several papers about the expansion factors Y for thin plates with a concentric hole but...
01 November 2020 3,472 4 View
Hi. I have the following experiment An independent-measures (between-subjects) quasi-experimental pre-post intervention (within-subjects) design was used with an intervention and control...
29 August 2020 8,375 11 View
Reflectance spectra are performed on the plane surface of a compact glass powder disk (5 mm thickness). The glass particles can range from 20 to 100 micrometers. The instrument is a portable...
21 July 2020 4,717 2 View
I have a table with data from one system which has several applications installed. The table contains the number of times the system has opened each application for each month. I would like to...
18 March 2020 9,656 4 View
I am trying to simulate the interaction between this acid and a protein, so I am following the steps of the protein-ligand Gromacs tutorial. However, I need a prm file to complete the complex...
27 November 2019 9,592 1 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...
15 July 2024 1,929 2 View
Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been...
11 July 2024 5,380 0 View
Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...
11 July 2024 8,484 0 View
I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...
07 July 2024 407 0 View
Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...
07 July 2024 7,967 3 View
I have seen some papers that differentiate C2C12 myoblasts into myotubes by changing the growth medium that contains 10%FBS by a medium containing 2%Horse serum/calf serum + 1% NEAA. What's the...
04 July 2024 4,732 1 View
I have tried to optimize the serum free dmem which is the best but i am afraid the secretory profile has too much variations.
03 July 2024 4,044 3 View