First division of sweet potato cells should occur after 5 days of incubation in liquid medium at 27°C in the dark and on average

Thank you!

We are also doing the same activities. For extraction, we are following preplasmolysis, digestion, floating and washing steps. Contineous dark will promote division of protopasts. l will update our progresses.

Thank you! Actually I have regenerated calluses from protoplasts, in liquid medium and dark conditions.