Does anybody have an idea how to sterilize gRNA for genome editing?

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Mahdi Moradpour

Hi Bessenyei

Your concern about sterilization of gRNA comes from which steps of CRISPR/Cas9 method?! And which kind of cells (plant, animal or!?)?

Are you asking about transformation step?

Enikő Besenyei

I do genome editing in potato protoplasts, and I am interested how can I sterilize the RNPs (Cas9 and gRNA) before transfection. Thank you!