Dear friends,
I run a FTIR spectrum of a drug and got the spectrum. I mixed with certain excipients and again run the spectrum.
The principle responses in the IR spectrum related to the drug structure were studied and I found that the response in wave numbers slightly vary between the pure drug and the drug excipient mixture.
For example pure drug produce a principle response at 1515cm−1 (for a particular group) the excipient drug mixture produce the principle response at 1530cm−1 (for the same group of the drug)
Is this variation is accepted?
If we run IR spectrum for 3 times for a same sample will it produce reproducible response over the wavenumbers in all the occations?
Is there any acceptance in such variations?
Thanks in advance
what is the nature of your sample? As you know there is an interaction between the functional groups with each other ,like dipole -dipole , H-bonding , ...etc these forces might the stretching frequency of bands changed or deviate .
Considering the figures you put in, yes this variation is accepted. However, as Mahrath mentioned: It depends on the vibrational excitation of the compound and this will certainly come down to its dependence on the nature of the bonds you have in your sample.
Thanks for sharing the answer.
There is one more query which you can share your thoughts.
The drug and the polymer will be interacted with lot of hydrogen bonding between them.
Is that the hydrogen bonding between the drug and the polymer will decrease the intensity of the peak? Will this hydrogen bonding affect the drug structure?
But content analysis by HPLC did not showed any interference and drug was recovered well.
I enclosed the FTIR Spectra for Drug, Polymer and Drug + Polymer
Thanks in advance
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