12 December 2017 2 6K Report

When picking single clones, I use cloning cylinders. A problem which I have faced is that when I use 100 uL of trypsin per cylinder, no cells adhere when directly transferred to a 48-well plate. I have inferred that this is because there is too much trypsin per unit media in the well. What is the optimal way to transfer from a cloning cylinder? Should I move the cells into a larger well? Or just use less trypsin?

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