What sentence do I have to put in the calculation entry to be able to make corrections for overlapping bases (BSSE)?

computational chemistry: I want to make the base overlap corrections for the dichloroacetyl chloride dimer. I put the following sentence to run the calculation in Gaussian: frec B3LYP/6-311++G**...

27 February 2020 6,347 3 View

What binding buffer would you recommend for NI-NTA superflow column purification?

I have to start the purification of a protein using a NI-NTA superflow column. My protein has an aproximate pI of 7. I have no experience with IMAC purification, i know i may have to try...

07 January 2020 3,884 4 View

Why do PC12 cells die after NGF addition?

I have been working with PC12 cells for years. Lately, however, I noticed that they start dying after 3 days of differentiation with NGF . My protocol is the following: I plate the cells onto poly...

02 January 2019 4,794 4 View

What are the best conditions for Western Blotting alpha synuclein?

I am working with PC12 cells and would like to know what are the best conditions to perform western blot of alpha synuclein (14 kDa). Especially, regarding: best buffer for protein extraction:...

08 May 2016 6,236 1 View

What is the best endogenous control for differentiating PC12 cells?

I am working with PC12 cells and I take mRNA samples every 48 hours after differentiation with NGF. Which would be the best endogenous control for qPCR given that it needs to be one that does not...

03 May 2016 2,054 1 View

What are duck egg patterns in inverse distance interpolation, and why it occurs?

25 April 2016 604 3 View

What makes Runge Kutta Method more accurate than Euler method for solving ODEs?

Runge Kutta method gives a more stable results that euler method for ODEs, and i know that Runge kutta is quite complex in the iterations, encompassing an analysis of 4 slopes to approximate the...

02 April 2016 5,304 18 View

What is the function of the ozone scrubber in some ozone analyzers that use UV absorption?

I understand the process of UV absorption, but I am not sure about the Ozone scrubber, is it just a matter of comparison, like a blank for the final result of ozone concentration in air?

22 January 2016 3,473 4 View

What is/are the best agent/s and protocol to differentiate SH-SY5Y cells into dopaminergic neurons?

I want to test effects of a toxic drug on SH-SY5Y cells and I read that there are many agents that induce differentiation into different phenotypes but I could not conclude which of them are...

28 April 2015 754 2 View

Can anyone suggest a protocol for EDS (Ethane dimethane sulphonate) preparation?

04 March 2015 8,537 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View