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Questions related from Luciana Sampieri
I would like to implement a endogenous RNA-based internal amplification control for a RT-qPCR assay used to detect viral pathogens in human plasma samples. Are there any detectable endogenous RNA...
23 May 2024 1,941 1 View
I am working on a multiplex qPCR to detect simultaneously a viral RNA target and an exogenous RNA internal control using hydrolysis probes. As you can see from the picture, this internal control...
02 February 2024 2,499 0 View
I have been working with PC12 cells for years. Lately, however, I noticed that they start dying after 3 days of differentiation with NGF . My protocol is the following: I plate the cells onto poly...
03 January 2019 4,968 4 View
I am using KT5720, a PKA inhibitor. The first time I used it, it had no effect at 1 uM 1 hour before my experimental treatment in PC12 cells. I would like to know what is the optimal time of...
19 September 2017 7,848 1 View
I am working with PC12 cells and would like to know what are the best conditions to perform western blot of alpha synuclein (14 kDa). Especially, regarding: best buffer for protein extraction:...
09 May 2016 6,419 1 View
I am working with PC12 cells and I take mRNA samples every 48 hours after differentiation with NGF. Which would be the best endogenous control for qPCR given that it needs to be one that does not...
04 May 2016 2,221 1 View
I want to test effects of a toxic drug on SH-SY5Y cells and I read that there are many agents that induce differentiation into different phenotypes but I could not conclude which of them are...
29 April 2015 951 2 View
I am trying to perform western blots of alpha synuclein (14 kDa), using wet transference 1h 15min at 85 V, but I get this weird "patchy" pattern, the band isn't a whole defined band. What could be...
22 April 2014 9,962 14 View
