Luciana Sampieri

10 Questions 8 Answers 0 Followers

Questions related from Luciana Sampieri

How can I improve recombinant Factor IX stability in aqueous solution during purification?

We are currently working on purification of human recombinant Factor IX (rhFIX) using three independent chromatographic steps. We experience significant loss of activity during purification and...

23 April 2025 7,254 1 View

Is UV-induced ozone harmful ?

Where I work, there are three separate rooms for setting up PCR reactions. In room number 2, whener we use UV light for decontamination, there is a particular odor that remains for minutes after...

12 August 2024 9,671 5 View

Can I detect endogenous RNA in human plasma samples?

I would like to implement a endogenous RNA-based internal amplification control for a RT-qPCR assay used to detect viral pathogens in human plasma samples. Are there any detectable endogenous RNA...

23 May 2024 1,947 1 View

How can I re-design a hydrolysis probe to avoid a non specific signal?

I am working on a multiplex qPCR to detect simultaneously a viral RNA target and an exogenous RNA internal control using hydrolysis probes. As you can see from the picture, this internal control...

02 February 2024 2,518 0 View

Why do PC12 cells die after NGF addition?

I have been working with PC12 cells for years. Lately, however, I noticed that they start dying after 3 days of differentiation with NGF . My protocol is the following: I plate the cells onto poly...

03 January 2019 4,972 4 View

How much time to treat PC12 cells with KT5720?

I am using KT5720, a PKA inhibitor. The first time I used it, it had no effect at 1 uM 1 hour before my experimental treatment in PC12 cells. I would like to know what is the optimal time of...

19 September 2017 7,854 1 View

What are the best conditions for Western Blotting alpha synuclein?

I am working with PC12 cells and would like to know what are the best conditions to perform western blot of alpha synuclein (14 kDa). Especially, regarding: best buffer for protein extraction:...

09 May 2016 6,424 1 View

What is the best endogenous control for differentiating PC12 cells?

I am working with PC12 cells and I take mRNA samples every 48 hours after differentiation with NGF. Which would be the best endogenous control for qPCR given that it needs to be one that does not...

04 May 2016 2,225 1 View

What is/are the best agent/s and protocol to differentiate SH-SY5Y cells into dopaminergic neurons?

I want to test effects of a toxic drug on SH-SY5Y cells and I read that there are many agents that induce differentiation into different phenotypes but I could not conclude which of them are...

29 April 2015 955 2 View

Can you help me figure out my problem with alpha synuclein Western Blot?

I am trying to perform western blots of alpha synuclein (14 kDa), using wet transference 1h 15min at 85 V, but I get this weird "patchy" pattern, the band isn't a whole defined band. What could be...

22 April 2014 9,968 14 View