Hi,

I have to do Hydroxyproline assay study. After the hydrolysis step, it is said we have to keep for vacuum drying.

As I do not have a vacuum dryer in our lab, I am planning to go for drying in oven at 60 degrees.

Can anyone suggest me how much time I should keep at that temperature when my sample volume is 10ul in 96 well plate?

Thank you,

Harsha

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