Hi,
I have to do Hydroxyproline assay study. After the hydrolysis step, it is said we have to keep for vacuum drying.
As I do not have a vacuum dryer in our lab, I am planning to go for drying in oven at 60 degrees.
Can anyone suggest me how much time I should keep at that temperature when my sample volume is 10ul in 96 well plate?
Thank you,
Harsha