How I can solve the overlapping problem between Sucrose and Raffinose in HPLC ?

I prepared the standard solution for all sugars and got the specific RT for each sugar (Raffinose 7.8 and Sucrose 8.4). And I found Raffinose is high in leaves bcz its stress experiment but the...

02 March 2019 9,183 5 View

Which method is better to extracting soil solution for measuring the EC in the soil?

I am creating artificial salinity by adding 150mM and 200mM (w/w) to the soil and after this I want to measure the EC in the soil and I have found different methods to extract the soil solution...

05 June 2018 1,967 9 View

Plz can you help me to download this book: Australian laboratory handbook of soil and water chemical methods 1992?

This book will help me in my research work

05 June 2018 4,012 0 View

Doing research or handling research is the right term?

Is it important to learn, how to do research? or how to solve the problems that you will meet while doing your research?

01 January 1970 1,273 8 View

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For people who are working in science, how you can solve that problem about feeling lazy after doing some work or finish one point in your "list to do" ? Is this because you feel better when...

01 January 1970 3,578 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

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03 March 2021 5,360 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View