I´m growing Scenedesmus sp. and Chlorella sp. inoculum in a f2 culture medium opened in 2012. It's possible that this issue interfere in the growing rate of the microalgae? I noticed that these solution include vitamins, it's possible these vitamins expired?
Thanks for all the answers.
f/2 is typically a seawater medium and Scenedesmus and Chlorella are freshwater algae - that is likely the problem.
You can continuing using the F2 Gillard Medium, you just need to autoclave the solutions with macronutrients and filter by Millipore the micronutrients such as the vitamins. hopefully this answer will be helpful for you.
best regards,
Laura Torrentera
The sterility and stability of the stock solutions of the f/2 media mainly depends on the storage of the stocks after initial opening. The stocks should be stored in refrigerated conditions after opening to prevent complications in the growth of the microalgae.
And as u rightly mentioned the media contains vitamins and certain trace metals that seem to precipitate and coagulate when stored under improper conditions which hinders the overall growth of the microalgae. I had faced this problem earlier during the cultivation of my marine Chlorella sp.
Hope this answer is helpful.
Regards
Aradhana S
Curtis is correct. Try switching the chlorella media to a 3N BBM media
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