How much time gap between DO fixation and titration is acceptable in case of DO determination by Winkler method ? (In the case of sampling site far from lab causing delay in titration)
In the Winkler method for dissolved oxygen (DO) determination, ideally, the titration should be performed as soon as possible after sample collection and fixation to ensure accurate results. However, if there is a delay due to the sampling site being far from the lab, the samples can still be stable if handled correctly.
Here are some guidelines for acceptable time gaps and precautions:
Storage Time: Samples can typically be stored for up to 6–8 hours after fixation without significant loss of accuracy. If delays are longer than 8 hours, the sample might lose some DO, leading to inaccurate results.
Storage Conditions: To preserve sample integrity, keep the fixed samples cool and away from direct sunlight. Ideally, store them at around 4°C in a dark container to minimize any potential changes. Avoid shaking or agitating the sample to prevent introducing or consuming oxygen.
The time gap between DO fixation and titration should not exceed 2 hours to ensure accurate dissolved oxygen measurements. Delays beyond this period may lead to changes in oxygen levels due to biological or chemical activities.