How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Why do I see variability in mRNA expression in qPCR of the same gene (ubiquitin)?

Hi everyone! I use HaCaT cells and i transfect them with siRNA. After 48 h I extract proteins with Laemmli and RNA with trizol. I proceed with western blot to check proteins levels (and they are...

26 January 2021 5,738 3 View

Why my IP injcetion drug for mice turn opaque after thawing?

Hi, I have prepared a drug solution that is dissolved in DMSO. We are giving IP injection to mice. The final stock concentration is 2mg/ml in 5% DMSO, 2% Tween-80 and 93% Saline. Before injection,...

18 November 2020 6,000 2 View

What protein about 100kDa is tyrosine phospholated when challenge T cell with anti CD3?

Hi, I uesd purified mouse CD3 T cell from spleen and stimulated these cells with anti mouse CD3 for different time. At least I can say two proteins are activated (black and red arrow). For the...

11 November 2020 7,202 3 View

Question on ELISA logistics with modified targets?

So, I am unable to find a direct answer but essentially I want to use an ELISA protocol to measure the amount of ubiquitylated histone H2A in my mutant cells. I was wondering if I could get a kit...

02 November 2020 6,761 2 View

Problem about rat anesthesia?

I have a problem about rat anesthesia, the situation is that I have Zoletil 50 1mg/ml + xylazine hydrochloride 23.32mg/ml solution that I prepare at the same time from the same source (from the...

07 October 2020 9,140 7 View

Incubation temperature/time required for IP with Pierce Anti-HA beads (Cat no: 88836)?

I am planning to perform immunoprecipitations using the Pierce anti-HA magnetic beads from Thermo Fisher (Catalog number: 88836) and I was wondering whether anybody has incubated these beads with...

05 October 2020 4,065 6 View

How do I run a western blot following co-ip?

This is the first time I do any sort of IP experiment and I was wondering if there is a certain procedure to follow for the western blot after the last elution step in the co-ip protocol. I am...

22 September 2020 3,688 3 View

MHCI IP for MS what detachment agent to use?

Dear colleagues, We are planning to run some MHCI IP experiments to identify MHC-bound peptide in adherent cells. I was wondering what do you usually use to detach the cells? I found accutase as...

26 August 2020 2,931 1 View

Best way to confirm phospho-mutants?

We have generated a few phosphomimetics mutants of our protein (constitutively active threonine and serine site). We are wondering about what is the best way to confirm the mutants have active...

25 August 2020 4,000 2 View

IP samples run weirdly on Western Blot?

Hi, Recently I have been doing IPs and WB afterward. But I have problems with many of my samples. Namely, samples run weirdly on the gel, they can not concentrate on one band as usual, and when I...

14 June 2020 6,747 8 View