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Questions related from Dipanwita Sadhukhan
I am trying to clone 5'UTR of a gene in pGL3 basic vector. I have done PCR of the construct and getting positive result. Howerver, when I digest my clone with the enzymes that were used to create...
20 February 2017 987 3 View
I want to characterise ubiquitination property of a protein. For that Ip assays will be done in presence of His-Ub. Do you please suggest me the amount in 100 mm dish. initially I transfected with...
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I am trying to do an imaging experiment where I need to observe cellular organelles. I do not have poly L lysine in my lab. My question is whether this line grow on coverslip with out coating?
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For confocal microscopy we are trying to capture signal next day of staining. But getting very weak and nonspecific staining.
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