Is anyone aware of any protocol for measuring cross section of muscle fibers which are fixed with 4% formaldehyde in 0.1 M phosphate buffer, pH 7.0, for 24 h at 4 °C , basically would like to know how much shrinkage correction should be done for the measurement ? since we will compare it with unfixed control samples
Hi Farhan,
it is possible using Image J + macro Macro_seg_5_modif.ijm.txt, you must label in immunofluorescence myofibers by laminin then acquire images and analyze these with ImgaeJ software. In attachmente you'll find the methods in the pdf file.
good luck
Hi Cesare Gargioli,
I am trying to use your suggested macro and method above. Can you please advise how to access the macro you suggest?
Thank you,
Deirdre
Hi Deidre,
in attachment you'lll find the macro, following the pdf file attached in my previous mesage you can make it work.
Good luck
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