I am planing to extract total phenolic compounds from seeds of buckwheat. But I am confused that how much seeds are enough for the total phenolic compounds leading to the characterization of each phenolics. Please give me suggestion about the weight is required and which procedure I should prefer for extraction. If someone has supporting material please share it.
Thank You
you can use 0.5 g and more
this enough
Article Chemical Composition and Antioxidant Capacity of Eggplant Pa...
The weight of the seed you need depends on the volume of the extract you want and what else you want to do with it. If it's only for characterization you have to consider the type of analysis you want carry out. Usually, GC-MS requires just 1microlitre of your extract.
The amount of the required extract depending on the amount of the dried plant.
You can take 200 gm of the dried plant an d follow the following extraction process ( Cannell,R. 1998, Natural products isolation- Humana Press, Totowa, New Jersey):
1-200 gm in 1000ml n-hexane..... work up
2- Extract the residue with 800 ml Methanol...work up
3- Extract the residue with 70% methanol....Work up
Take TLC for all......separate the required compounds by column or flash chromatography.......
You can go for 5 gm of dried fine mesh powder of your plant with 100 ml of methanol and leave of over night. you can used the technique of column chromatography for separation and then go for HPLC to obtain more pure compound.
( Soni N, Singh DK, Singh VK. HPLC characterization of molluscicidal component of Tamarindus indica and its mode of action on nervous tissue of Lymnaea acuminata. J Ayurveda Integrative Med. {online first), 2017. 1-11 Elsevier. IF-0.98.2018)
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3rd Mar, 2020
Duduzile Molefe-Khamanga
Sadu Scientific Analysis and Research Consultant
Depending on which organic solvent you will use for extraction. And again you will still need to purify the residue so that you will get the that phenolic compounds. Sequntial extraction is the most recommended method. Whereby you wash seeds of buckwheat with Hexane until no color is comes out when washing with Hexane. This means you have extracted the compounds that are soluble in non-polar compounds. Then the residue of the seeds of buckwheat which was washed with hexane must the washed with ethyl acetate or dichloromethane again until there is no colour coming out during the washind with EtOAc or DCM. Then the residue must be washed with the Ethanol. This is when the phenolic compounds will be extracted but there will be other compounds too. Hence the Ethanolic extract residue will need to be checked by GC-MS to find out the possible phenolic compounds of interest. Then purified further. Where you must look for the the suitable mobile phase. So it is a processes.
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1 Recommendation
6th Mar, 2020
Naser Jawad Kadhum
University Of Kufa
you can use 0.5 g and more
this enough
Article Chemical Composition and Antioxidant Capacity of Eggplant Pa...
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6th Mar, 2020
Ahmed Madhloom
University Of Kufa
good answer Naser Jawad Kadhum
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