Is there a way to calculate ddCts/fold changes if your gene of interest is undetectable in unstimulated but then detectable after stimulation?

Hi all This must have happened to others before. For example an IFN stimulated gene like MX1 goes from undetectable (>40 cycles) but then after IFN alpha stimulation the Ct is now 28. Both...

26 March 2018 9,347 4 View

What is the best way to measure virus specific IgG from in vitro human cell stimulations?

We are setting up a variety of virus stimulations as positive controls for adding to human PBMCs. My goal is to measure IgG induced by the virus so while total IgG is easy to measure via ELISA,...

23 June 2014 2,463 4 View

What is the fate of IT staff after adopting the cloud?

03 April 2014 5,153 5 View

If an organization decided to migrate to the cloud, what is the roadmap for a successful migration?

If an organization decided to adopt cloud computing services, is there any specific procedure to go about migrating?

03 April 2014 6,116 6 View

Has anyone been successful using filter plates to surface stain/wash human PBMCs for flow cytometry?

I am curious if anyone has been successful using a vacuum manifold to wash FACS samples instead of centrifugation to save lots of time if stain samples in a 96 well millipore filter plate? We...

24 March 2014 9,316 5 View

To cloudify or not to cloudify: What are the most influential factors in deciding to move or not to move to the cloud?

If an organization wishes to migrate to the cloud what things need to be taken into consideration before such move takes place?

02 March 2014 506 7 View

What is the journal impact factor for e-Health publications now?

This question warrants a response from the general medical and academic community since this is a new genre of medicine that is building exponentially in impact on health care services nationwide.

01 January 1970 5,456 5 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View