I want to treat crude oil contaminated soil using Aspergillus niger. I also want to use particles of periwinkle shell and saw dust to improve the texture of the soil and loosen it somewhat. The spores have already been grown and ready for use. However I have the following questions.

1. How much of the Aspergillus niger do i need to inoculate the soil for treatment?

2. How best do I go about the inoculation process?

3. How do I measure the quantity of spores?

I would be grateful for some suggestions. Thanks.

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