How much neutralizer (For example tween 80) should be added to the culture medium to neutralize the antibacterial on the product?

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C K Gomathy

Hi.,

A neutralizer is added at the end of the contact time ... new antimicrobial efficacy methods including this one was carried-out from 2007 to 2009 in .... For this test organism, the culture medium should be prepared as follows: - prepare .... contact period, 10 mL* of a validated neutraliser (containing Polysorbate-80 as specified.

For more info:

https://aac.asm.org/content/19/3/429.full.pdf

Farzan Nabati

Thanks,

Dear Dr. Gomathy

hi,

thanks for your reply..

Abdelkader Mohamed Elsayed

I Agree With C K Gomathy