Hello Syed,

You are going forward too much! The best criterion for live sperm is motility. If sperm is immotile and live, what is the use? Not all modern or latest analytical technique is always the best. Sperm must show to fertilize the oocyte without any reasonable doubt. Swim-up method in 10ml culture tube definitely beat the negative selection of Anx5-based MACS separation. Only live sperm with high motility could arrive the site of fertilization in in-vivo and undergo a serious of metabolic events (called hyperactivation), gradual acrosomal exocytosis induced naturally by the zona pellucida protein, limited release of tiny amount of acrosomal enzymes to make its narrow path through the zona in both in-vivo and in-vitro.

Better option can be obtained from the practice of the swim-up procedure cleanly, repeatedly, developing your muscle and nerves in your performing hands and sharp eyes.

Cryopreservation Protocol should be optimized for this purpose

When spermatozoa are capacitated, PS are flipped. Spz then obviously become annexin V positive although they certainly are not apoptotic ! Annexin V therefore is certainly not a good tool for getting rid of apoptotic spz !