Hi,

I noticed a lot of variability in my TOP FLASH assay with CHIR99021 (2.5uM). In some assay, I see 100 fold increase compare to control and in other, I will detect a 1000 fold increase.

I'm transfecting 8x104 HEK293T cells with 297 ng M50 STF plasmid + 3ng pRL (renilla plasmid) per well and I'm using Promega Dual Glow Luciferase kit.

Thank you for your inputs.