Mathieu Derouet

6 Questions 4 Answers 0 Followers

Questions related from Mathieu Derouet

How much fold increase you observe with CHIR in your TOP FLASH assay ?

Hi, I noticed a lot of variability in my TOP FLASH assay with CHIR99021 (2.5uM). In some assay, I see 100 fold increase compare to control and in other, I will detect a 1000 fold increase. I'm...

10 October 2023 8,844 0 View

Does anyone ever used RNAscope® H202 & Protease Plus Reagents past their expiration date ?

Hi, I'm looking to learn how to use RNAscope. I could have access to some of the reagents but they are expired. Since the reagents and probes are quite expensive, I was wondering if I could use...

26 January 2023 5,317 2 View

Which IHC marker can we use to differentiate gastric cancer from normal gastric tissue ?

I have some parraffin blocks with cells extracted from gastric cancer patient and healthy patients. So far, I did not find a marker which was exclusively expressed in gastric cancer. Does anybody...

25 August 2016 8,920 3 View

Could you help me identify these cells isolated from a lung cancer xenograph ?

These cells were isolated from lung cancer xenograph. I have been culturing them in DMEM/F12/B27/FGF for over a month now. They looked like fibroblasts to me but they are forming colonies. Thank...

05 March 2015 1,934 5 View

Is there any scenarios where cells that have a higher ability to invade can posses a higher adhesion potential?

30 April 2014 3,712 4 View

Stable tumor suppresor miR clones

Is it possible to obtain a tumor suppressor resistant population from stable transfection with a tumor suppressor miRNA? If the miRNA is supposed to slow proliferation and induce apoptosis, then...

15 January 2013 3,750 3 View