How much fat tissue a person needs for normal hormonal functioning of adipokines such as leptin and others?

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During exercise, would increased pH in the sarcoplasma lead to a different flux of H+ with lysosomes, as the pH in the cytosol will decrease?

During exercise, would increased pH in the sarcoplasma lead to a different flux of H+ with lysosomes, as the pH in the cytosol will decrease? During exercise the pH can decrease due to protons...

01 February 2020 2,045 1 View

Would resistance training lead to a form of neurogenesis and changes of motor unit innervation of muscle cells?

Would resistance training lead to a form of neurogenesis and changes of motor unit innervation of muscle cells? So RT can lead to alteration of the nervous system and creation of new branches or...

06 July 2018 4,028 0 View

Can we scientifically rate veganism?

Can we scientifically rate veganism? 1) It is claimed that DHA led to better functional brains. This would be important in the human developement. (is this correct?) 2) DHA is nowadays (what...

05 June 2018 2,570 0 View

Concurrent training. Would type IIx muscle fiber, not contain more myoglobin because it can be reached less efficient by oxygen because of fiber size?

Concurrent training. Would type IIx muscle fiber, not contain more myoglobin because it can be reached less efficient by oxygen because of fiber size? Would bigger muscle fibers that are hard to...

05 June 2018 7,177 0 View

In case of omega 3 supplementations and training effects, should there first be a ratio of 1:5 omega 3 to omega 6 before we can assess real effects?

In case of omega 3 supplementations and training effects, should there first be a ratio of 1:5 omega 3 to omega 6 before we can assess real effects? The ratio n-3 to n-6 is not balanced. This...

04 May 2018 9,030 1 View

Is there literature about power lifting and weight lifting and the hamstring curl or knee flexor capabilities?

is there literature about power lifting and weight lifting and the hamstring curl or knee flexor capabilities? Most they do squat, deadlift, goodmorning, straight leg deadlift, but it seems the...

04 May 2018 3,460 2 View

Do we need white adipose tissue to a large extend on basis of hormons?

do we need white adipose tissue to a large extend on basis of hormons? Vitamins can be stored in IMTG, or in beige/brown adipose tissue. Energy we do not need with normal food stores. Isolation...

04 May 2018 3,532 2 View

Is sport science about individual holistic approach, or inter-individual reductionistic specialism?

Is sport science about individual holistic approach, or inter-individual reductionistic specialism? Should someone try to know as much as possible about everything related to the wide field of...

04 May 2018 4,082 5 View

Should the pectoralis major pars abdominal be trained with a different insertion position as the pectoralis major pars sternum and/or clavicula?

Should the pectoralis major pars abdominal be trained with a different insertion position as the pectoralis major pars sternum and/or clavicula? The insertion is different for the three parts. So...

04 May 2018 2,877 0 View

In shoulder press and lats pulldown there is the "breaking the bar" principle to limit injury. Is this correct, and/or are there other options?

In shoulder press and lats pulldown there is the "breaking the bar" principle to limit injury. Is this correct, and/or are there other options? This to create external rotation. Is there any...

04 May 2018 7,837 2 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

Why is this tissue slice different?

This is a slice of intestinal tissue from the Chinese mitten crab (intestine in the upper right corner). I injected WSSV into this horseshoe crab. Now I want to know what these red spots next to...

18 July 2024 5,602 0 View

Can we progress towards defining obesity based on body fat content?

The burden of obesity is enormous. The greater challenge lies in accurately diagnosing obesity at the right time, reflecting the true increase in visceral fat. BMI may not accurately represent...

14 July 2024 8,771 4 View