During exercise, would increased pH in the sarcoplasma lead to a different flux of H+ with lysosomes, as the pH in the cytosol will decrease?

During exercise, would increased pH in the sarcoplasma lead to a different flux of H+ with lysosomes, as the pH in the cytosol will decrease? During exercise the pH can decrease due to protons...

01 February 2020 1,839 1 View

Would resistance training lead to a form of neurogenesis and changes of motor unit innervation of muscle cells?

Would resistance training lead to a form of neurogenesis and changes of motor unit innervation of muscle cells? So RT can lead to alteration of the nervous system and creation of new branches or...

06 July 2018 3,844 0 View

Can we scientifically rate veganism?

Can we scientifically rate veganism? 1) It is claimed that DHA led to better functional brains. This would be important in the human developement. (is this correct?) 2) DHA is nowadays (what...

05 June 2018 2,408 0 View

Concurrent training. Would type IIx muscle fiber, not contain more myoglobin because it can be reached less efficient by oxygen because of fiber size?

Concurrent training. Would type IIx muscle fiber, not contain more myoglobin because it can be reached less efficient by oxygen because of fiber size? Would bigger muscle fibers that are hard to...

05 June 2018 7,008 0 View

Is sport science about individual holistic approach, or inter-individual reductionistic specialism?

Is sport science about individual holistic approach, or inter-individual reductionistic specialism? Should someone try to know as much as possible about everything related to the wide field of...

04 May 2018 3,917 5 View

In case of omega 3 supplementations and training effects, should there first be a ratio of 1:5 omega 3 to omega 6 before we can assess real effects?

In case of omega 3 supplementations and training effects, should there first be a ratio of 1:5 omega 3 to omega 6 before we can assess real effects? The ratio n-3 to n-6 is not balanced. This...

04 May 2018 8,842 1 View

Is there literature about power lifting and weight lifting and the hamstring curl or knee flexor capabilities?

is there literature about power lifting and weight lifting and the hamstring curl or knee flexor capabilities? Most they do squat, deadlift, goodmorning, straight leg deadlift, but it seems the...

04 May 2018 3,286 2 View

Do we need white adipose tissue to a large extend on basis of hormons?

do we need white adipose tissue to a large extend on basis of hormons? Vitamins can be stored in IMTG, or in beige/brown adipose tissue. Energy we do not need with normal food stores. Isolation...

04 May 2018 3,299 2 View

Should the pectoralis major pars abdominal be trained with a different insertion position as the pectoralis major pars sternum and/or clavicula?

Should the pectoralis major pars abdominal be trained with a different insertion position as the pectoralis major pars sternum and/or clavicula? The insertion is different for the three parts. So...

04 May 2018 2,708 0 View

In shoulder press and lats pulldown there is the "breaking the bar" principle to limit injury. Is this correct, and/or are there other options?

In shoulder press and lats pulldown there is the "breaking the bar" principle to limit injury. Is this correct, and/or are there other options? This to create external rotation. Is there any...

04 May 2018 7,656 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View