I am studying on HIV and I wonder "Do antibodies destroy HIV?".
If yes, how it happens? If no, then can just one HIV cause AIDS?
Thank you.
The wire/cable in my chamber (for protein electrophoresis) was cut in half. I tried to re-join it by knotting the two ends. But the current still run unevenly among running wells. I want to ask...
07 August 2019 9,588 8 View
I have a viral linear DNA sample extracted from an ocular washing solution. I want to run sequencing for its two genome ends (under 1kb each and I have known the sequence of the rest of its...
03 April 2018 10,011 5 View
I have a PCR product sample which was extracted about 1 year ago. I tried to electrophoresis it and a bright band appeared! Then I diluted it 9 times, 4 times and 2 times into 3 tubes and...
01 February 2015 2,565 4 View
I am a student and I am studying on Prokaryote's genome. I wonder if circular DNA in Prokaryote proved? Thank you!
11 December 2014 9,528 7 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...
18 July 2024 4,183 2 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...
15 July 2024 6,008 5 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View