This is my new project research work upon "microorganism isolation from food sample using nanomagnetic particles". Briefly, first of all, MBs(magnetic beads) were coupled with anti-Yersinia antibody. Then, antibody-conjugated beads were used for isolating bacteria from food sample. After that, to separate the bound bacteria on IMBs, elution buffer was effectively applied. In this situation, can I use this IMBs ( immunomagnetic beads) for again isolating bacteria from food sample without any coupling with antibody solution?.
The question is difficult to answer. Most affinity materials can be reused to some extent. However, it depends on your conjugation method (covalent?), type (polyclonal, monoclonal) and affinity of antibodies, and the kind of elution buffer (some buffers denature the antibody completely). In general, your question only can be answered experimentally. If you should consider a reuse, you need a parallel positive control in any case!
Thank you for your answer. I used polyclonal antibody and followed the procedure provided by the supplier company for coupling the beads with antibody. The elution buffer was glycine buffer, pH=3 for eluting the bacteria attached to the beads.
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