How many times can Fast Green Solution be re-used? How long can it be stored for before re-use?

10 February 2021 0 444 Report

I am doing some TRAP staining of rat long bones, and using Fast Green as an alternative to haematoxylin for the counterstain.

I am making up 0.08% Fast Green (Sigma 68724 - UK) w/v in 250ml water for my solution.

Can I re-use this solution?

If I can, how many times?

Do I need to store it in any special way in between staining sessions? How long will the solution keep?

Thanks very much,

Flynn

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Science method

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