Hi Flynn: Yes this is absolutely fine for the majority of antigens. Keep your dehydrated bones in the fridge to preserve them and make sure they do not dry out during scanning. We used it routinely for papers including Galea GL et al J Biol Chem 2014, Meakin LB et al J Bone Miner Res 2014 and many others.

Thank you for thinking of ways to reduce animal use! Also have a look at our Site Specificity screening tool (Galea GL et al Methods Mol Biol 2021) as a way to identify highly-responsive bone sites to direct your histological analysis if relevant.

Hello Flynn, Yes a agree in undecalcified hard tissue histology this is routinely done. One tip you can use sponges to hold samples in place so the images are better. We sometimes also scan the samples when they are in resin. Good luck!

Uwe Yacine Schwarze Gabriel L Galea

Thanks very much! Really helpful, I'll pass this on.

Kind regards,

Flynn

Not suprisingly that micro CT and Histology can be used simultaneously at the same bone to complement each other , especially at the thoracic rib fractures. Though Micro CT scan is said less sensitive to soft tissues,But is deemed sensitive to sanitize ocult/hidden pathology where the Histology can not pick it correctly.However there's limited level 1 study evidences to validate which one is superior over the other.

Ref:https//doi.org/10.1016/j.forsciint.2019.01027

Thanks very much for your response Daniel Gululi

Please note that if you are interested in absolute mineral density of your samples (running phantoms for calibration) formalin can have a slight decalcifying effect over longer time spans. So make sure that those 2 days does not become months/years.