Flynn Lachendro

6 Questions 5 Answers 0 Followers

Questions related from Flynn Lachendro

Quantifying Osteoblasts Using H&E Staining?

I am trying to perform osteoblast quantification using H&E. I have been reading papers (like this one: Extracellular matrix with defective collagen cross-linking a... ) where measurements like...

06 May 2021 2,142 2 View

How many times can Fast Green Solution be re-used? How long can it be stored for before re-use?

I am doing some TRAP staining of rat long bones, and using Fast Green as an alternative to haematoxylin for the counterstain. I am making up 0.08% Fast Green (Sigma 68724 - UK) w/v in 250ml water...

10 February 2021 426 0 View

Is it possible to perform microCT and then histology on the same bone?

Myself and my colleagues are thinking about maximising our small supply of lab rats, and so (with some helpful external information) we have been thinking about fixing femurs and tibiae in 10%...

04 February 2021 2,693 6 View

Help with Rat Long Bone Histological Preparation?

Hello everyone, I am looking to perform histology and immunohistochemistry on rat long bones (mainly femurs and tibiae). I would like some expert help with my preparation protocol for the...

01 October 2019 7,445 4 View

Is it possible to perform IHC / histology on bones which have been fixed in formalin and then CT scanned?

I am trying to couple angiogenesis and osteogenesis in a rodent model of spinal cord injury. What I would like to do is take a bone which exhibits osteoporosis (bone loss) and couple this with the...

10 April 2019 1,971 3 View

Is it possible to perform IHC / histology on bones which have been fixed in formalin and then CT scanned?

I am trying to couple angiogenesis and osteogenesis in a rodent model of spinal cord injury. What I would like to do is take a bone which exhibits osteoporosis (bone loss) and couple this with the...

04 April 2019 6,347 4 View