See attached pdf. All the best, Charles VINCENT

Thank you so much Doctor.

Hi, Nichioua Zakariya

Study this link:

https://www.nap.edu/read/11523/chapter/4

Thank you so much Samaneh Mahmoudvand.

Dear Nchioua

Interesting, maybe useful, information here on statistical power and study design.

Good luck!

https://www.nc3rs.org.uk/experimental-designstatistics

Dear Nchioua Z.

The number of rats may depend on the type of toxicity test (acute of long term) you 're intending. it may depend also on the protocole method (up and down or OECD etc... ).

Hi,

For toxicological studies specially on fungicide refer guidelines : OFFICE OF PREVENTION, PESTICIDES AND TOXIC SUBSTANCES ( ORAL OPPTS 885.3050) also refer OECD 423 acute toxic class method..

Thank you so much, your answers were helpful, thank you so much.

Hi Nchioua...

All the best

10 animal in each group used

LD50 test about 10 rats

Toxicological signes 3-4 does about 24-30 rat

The Up-and-Down procedures for oral toxicity begins with 3 rats and dose 2000mg/kg. Any case - use the OECD guidelines