Hi everybody,

I would like to ask you how many pmol should I use when I run in vitro transcription? I use as template purified pcr products with adapters - T7 promoter sequences. Is it true that it´s more beneficial to use lower pmol of DNA (0,5 pmol) for longer fragments (800 bp) and higher pmol of DNA (1-2 pmol) for shorter fragments (200 bp)?

Thanks for your references.

Sincerly,

Matej Medla