Do you think we can monitoring special material in plants via live organism as a bioindicator?

for example some bee can recognize some gums on plants and can know them from other kinds of gum

09 October 2016 2,736 7 View

Do you think dodder substances are changed according to it's host?

some traditional material are obtained from dodder, orbanch, etc. are these things change by changing host?

07 August 2016 3,653 0 View

Do you know how long a new variety has to preserve it's ability?

we know each variety lose some characters during years. some of these traits are very important for us, such as yield, essence, resistance to pests, etc. how long are these characters fix?

07 August 2016 4,528 6 View

Can parasites can change quantity of plant substances?

07 August 2016 4,887 0 View

Do you know anything about leech and leeching?

i need scientific and modern method.

31 December 2015 8,809 3 View

Are there any standard methods for producing or marketing of leech?

31 December 2015 7,998 3 View

Is there any validated information about stones effect on human health?

31 December 2015 2,293 3 View

How many plants are there in the world? do you know valuable data?

"250000-300000 species" is correct?and what about cultivars/varieties?

10 November 2015 9,101 2 View

What is Orthodox medicine?

Herbal and orthodox medicine

10 November 2015 2,233 3 View

Is the color of essential oil a good guide?

can we provide a descriptor for essential oils color in one family/genus?

08 September 2015 5,700 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Hello house, is it necessary to compute Content Validity Ratio (CVR) for an adapted questionnaire pls?

What should one do if he obtains conflicting results between Content Validity Index (CVI) and CVR for items in a quetionnare. For example based on the CVI obtained the items are acceptable but the...

25 February 2021 7,325 2 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What is this fern?

Please help me to identify this fern species. Thank you in advance.

24 February 2021 2,435 5 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View