I am trying to characterize PBMC from mouse blood.
1-3 mil, depending on the accuracy of your method
Sadly its a very small number! To get usable amounts I would suggest you pool several mice or flow whole blood to avoid massive loss due to the buffy-coat step. It works relatively well! Sorry I don't have better news.
Elisa A Liehn Thank-you very much, I am able to isolate approximately 1 million per ml.
Can You please suggest a method to characterize the PBMC?
I am trying it with Turk's staining method.
Any suggestions?
Thanks again.
Matthew Stephens Thank-you, your suggestion is really very helpful.
This strongly depends on the donor / source.
I am characterize them by flow cytometry using multiple panel (CD45, CD11b, Gr1, CD3, CD4, or add CD34 etc.), depending of interest.
almost one millions
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