How many KO mice do you get per litter for your UCP1 KO mouse model?

19 October 2018 0 4K Report

I am just starting to breed Het x Het at 27 degrees C and only got 10% KO mice. From 3 breeding pairs I got 19 mice, and 2 are KO's. Using B6.129-Ucp1tm1Kz/J from Jackson. Any advice to increase KO breeding efficiency would be greatly appreciated. Thanks!

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