How many Chromosomes are there in this picture?

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Gregory Dressler

It is hard to tell, but you should know that any established cell line, including Hela cells, has a variable number of chromosomes. So to get a measure of the distribution you must look at many spreads, not just one. According to the ATCC the range of chromosome number for Hela C2 cells is 70-162 with a mode of 82 !

Yuliia V. Martynova

This metaphase plate has too much overlays of chromosomes. In addition, in view of previous author's message about the instability of the chromosomes number in HeLa line, it is extremely difficult to understand, what chromosomes are overlapping. To output we need to analyze 50-100 plates, check out at least a few. If the majority of metaphase plates will have overlays, try to drop the cell on to slides from a greater height.

Halil Erhan Eroğlu

Dear Sura,

It is very difficult to give the exact number of chromosomes on this picture. It should be evaluated on at least 20 plates.

Yuksel Coskun

It is not easy to give exact chromosome number since many chromosomes are overlapped. It is better to check many plates.

Wojciech Witarski

I agree with opinions above, such a wide spread might indicate some inaccuracies due to preparation; I would go for a nice grouped ones (possibly non-overlapping:)).

Ram J Singh

I feel, cell is incomplete and has few chromosomes outside of the cell. I suggest to retry making new chromosome spread and examine several cells with well spread chromosomes. Please try 8hydroxyquiniline as a pretreatment.

Sonal Rajiv Bakshi

The question implies many things here. You can be confident that what you can not count, others will also not be able to count, if anyone answers any number, no need to believe it! One needs to work on many background aspects, rightly answered by Dressler and Martinova. Methods to optimize chromosome preparation, best source - AGT blue book and the recent video DVD [http://www.agt-info.org/Pages/agtstore.aspx] and spending reasonable time with cytogenetics lab, and offcourse knowledge about the cell line modal number etc. will make such a question redundant. Even a lab colleague would answer, go to a better metaphase cell. Have answered on a lighter note, but am really concerned about how we are doing information sourcing.