How many CHO cells must be seeded to be confluent after fixation for a 35 mm dish ?

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Quel est le mail de Aychew Kassie ?

Aychew Kassie

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Fang Liu

if for a imaging purpose, i suggest you seed at about 100,000/mL. 35 mm dish can contain 1-1.5mL

Ezequiel Wexselblatt

In a 24 wells plate I usually seed 100,000 cells in 0.2 mL and after 24 hours of incubation it does not fully cover the bottom but it is more than enough to analyze them by FACS. I believe that it should be adapted to the time you want to grow them and what to use them for.