Hello everyone! Please how to quantify well blood vessels with qupath software?

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Is it possible for the soil pH to be around 8, but with negligible amounts of carbonates, bicarbonates and exchangeable sodium?

Can this pH of 8 be due to the presence of other substances? Or do I have an error somewhere in the analysis? Thank you!

26 June 2024 9,093 4 View

What are the prices for the following antibodies?

BD Pharmingen™ FITC Mouse Anti-Rat αβ T-Cell Receptor (561672) BD Pharmingen™ APC Mouse Anti-Rat CD4 (550057) BD Horizon™ V450 Rat anti-Mouse CD8a (560469) BD Pharmingen™ PE Mouse Anti-Rat CD45R...

10 May 2024 3,072 0 View

What is the recommended sample size for the validation of a standard questionnaire consisting of 40 items?

Dear colleagues, I am looking for advice on the validation of a standard questionnaire that I intend to translate. The original version contains 40 items. Could you please tell me what sample...

26 March 2024 1,660 11 View

Why does a precipitate form rapidly when i add a solution (Nitric acid + water ) to a solution of TTIP in isopropanol to ?

So I have ben trying to prepare TiO2 nanoparticles but i am getting trouble with the fromation of White PPT which later dissolves to form a clear solution that unfortunately doesnt form a gel. it...

25 March 2024 8,808 2 View

Does antigen retrieval for IF on tissue samples need to be done on the same day?

For both heat-induced or proteolytic-induced antigen retrieval, does the AR needs to be done on the same day of the IF? Or can be done one day in advance, store the slides at 4º, and performe the...

06 March 2024 220 1 View

Any empirical study about theater education for immigrant students?

03 March 2024 6,134 0 View

How can I model a packer in Abaqus for segmental tunnel linigns?

Currently, I am trying to model packers made from recycled plastic material (E=360N/mm^2 and a poisson ratio of 0.4). The best way to model these packers would be to only adjust the contact...

26 February 2024 5,699 1 View

What is the pH and osmolarity to measure the activity of myeloperoxidase (MPO) from the formation of hypochlorous acid?

Optimal buffer and conditions to determine the enzymatic activity of MPO by the colorimetric method that is based on the formation of hypochlrous acid which reacts with taurine to form the...

07 February 2024 3,056 0 View

Can convergence (SCF) be improved for transitional metals?

In Guassian, which convergence algorithm is best for working with transition metal interaction? It is taking a long time to even get the first block of coordinates, in the output file it said to...

28 January 2024 4,276 1 View

What is the ideal microscope objective (20x or 40x) to assess the size of adipocytes in abdominal adipose tissue in a murine model?

I need to evaluate abdominal adipose tissue hypertrophy in a murine model with metabolic syndrome, with and without treatment and I need to review which is the best objective to measure adipocyte...

09 January 2024 7,149 0 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

Can you visualize platelets using EVOS ?

Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...

24 July 2024 9,337 3 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View

Prediction and quantification of water content ?

Hi all. Is it possible to correlate multiple parameters like fruit weight, color, length and diameter, its ratio with water content in coconut ?. Need some guidance on some non destructive methods...

23 July 2024 9,421 3 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

How can I decrease the error of Rietveld method for phase quantification?

Phase quantification in steel contain BCC and FCC

20 July 2024 8,150 7 View