I am trying to optimize assay sensitivity for molecular diagnostics from a fine needle aspirate biopsy, but I'm struggling to find good information on the total number of cells harvested from the procedure.
Dear James
This article enclosed may be useful for you
Quantitative analysis of fine needle aspiration biopsy samples
Of course it depends how cellular is the lesion one is aspirating. Take a fibroadenoma of breast not hyalinized shows marked cellularity but hyalinized shows very little cellularity. Total number of cells harvested from a procedure vary considerably by the experience of the aspirator, the technique, the cellularity of the lesion and the biology of the lesion. See Diagnostic cytopathology of the breast. I am sorry if this is not the answer you are looking for.
Prabodh Gupta MD University of Pennsylvania.
Besides being lesion dependent, number of passes, type of lesion, accuracy of sampling, representative cells, tumor necrosis, cystic change, scarring are some of the other contributory factors affecting the cell yield.
In most instance, 5 passes using a #23 needle should give sufficient material for most special studies.
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