Hi!
I'm trying to calibrate a phylogenetic tree using BEAST and secondary priors based on a previously published work. However, I was wondering about the effects of the number of calibration points chosen, because I've identified at least nine points useful for my analysis but I ignore if it may be exaggerated (my tree includes about 480 tips and 190 species).
Any advice or literature will be welcomed!
Hi Daniel,
When using secondary calibration points, it's highly recommended to use a few. But when you want to incorporate fossils or biogeographics events you should use as many as you can. This because when you implement secondary calibrations it is highly probable to incorporate errors and bias associated to the methodology used with the secondary calibration.
Here at the Institute of Biology-UNAM, there is a very good class on molecular systematics and a module focuses on dating analysis including calibrations. It is offered by Dr. Susana Magallón. I include some of his references in case they help you.
Article A metacalibrated time-tree documents the early rise of flowe...
Article A Review of the Effect of Relaxed Clock Method, Long Branche...
Article Land plant evolutionary timeline: Gene effects are secondary...
Article Using Fossils to Break Long Branches in Molecular Dating: A ...
Hope this will help you!!!!
Saludos.
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